AB0065 HGF/C-MET SIGNALING PROMOTE ANGIOGENESIS THROUGH CXCL16 IN RHEUMATOID ARTHRITIS
نویسندگان
چکیده
Background: Hepatocyte growth factor (HGF) binds to the receptor tyrosine kinase c-Met and is a multifunctional cytokine that promotes processes such as cell proliferation, survival, differentiation, migration angiogenesis [1]. We previously reported HGF produced by inflammation in RA synovium, activates monocyte synovium bone destruction through its own chemotactic effect enhanced chemokine production [2]. Objectives: Therefore, we next aimed determine role of angiogenesis. Methods: The expression / serum synovial tissues (STs) patients controls human umbilical vein endothelial cells (HUVECs) was evaluated ELISA immunostaining. HGF/c-Met signaling on promotion CXCL16 from HUVECs fibroblast-like synoviocytes (FLSs) determined ELISA. To examine angiogenesis, performed vitro Matrigel assays using treated with HGF. Results: treatment-naive significantly higher than treatment-resistant showed significant positive correlation CXCL16. were expressed vascular STs HUVECs. Stimulation dose-dependently increased production. signal inhibition SU11274 suppressed TNF-α stimulation-enhanced FLSs dose-dependent manner. Furthermore, induced HUVEC tube formation 1.8-fold. Conclusion: potent angiogenic synovium. These results indicate strategy targeting signalling may be important for resolving RA. References: [1]Nakamura T, Nishizawa Hagiya M, et al. Molecular cloning hepatocyte factor. Nature. 1989 Nov 23;342(6248):440-3. [2]Hosonuma Sakai N, Furuya H, Inhibition factor/c-Met abrogates joint suppressing rheumatoid arthritis. Rheumatology (Oxford). 2021 Jan 5;60(1):408-419. Disclosure Interests: None declared
منابع مشابه
Angiogenesis in rheumatoid arthritis
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ژورنال
عنوان ژورنال: Annals of the Rheumatic Diseases
سال: 2021
ISSN: ['1468-2060', '0003-4967']
DOI: https://doi.org/10.1136/annrheumdis-2021-eular.3491